Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/12 - Revision history

Vi Nguyen: /* November 12, 2012 */

2012-11-15T12:51:27Z

November 12, 2012

←Older revision		Revision as of 12:51, 15 November 2012
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==November 12, 2012==		==November 12, 2012==
-	* The PCR products were purified with the Zymo DNA Clean ~~and Concentration~~ Kit	+	* The PCR products were purified with the Zymo DNA Clean & Concentrator Kit

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Karmella Haynes: /* November 12, 2012 */

2012-11-13T23:33:32Z

November 12, 2012

←Older revision		Revision as of 23:33, 13 November 2012
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	** In a 30 μL reaction, cut H2B with XbaI and SpeI		** In a 30 μL reaction, cut H2B with XbaI and SpeI
	** In a 30 μL reaction, cut LOV with XbaI and SpeI		** In a 30 μL reaction, cut LOV with XbaI and SpeI
-	** In a 30 μL reaction, cut a pSB1A2 plasmid (from Rene) XbaI and SpeI	+	** In a 30 μL reaction, cut a [http://partsregistry.org/Part:pSB1A2 pSB1A2 plasmid] (from Rene) XbaI and SpeI

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Karmella Haynes: /* November 12, 2012 */

2012-11-13T23:25:15Z

November 12, 2012

←Older revision		Revision as of 23:25, 13 November 2012
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	\| LOV \|\| 0.048 \|\| 0.026 \|\| 1.860 \|\| 48.095		\| LOV \|\| 0.048 \|\| 0.026 \|\| 1.860 \|\| 48.095
	\|}		\|}
		+
		+
		+	----
		+
		+	Great! Next step...
		+
		+	* Digests:
		+	** In a 30 μL reaction, cut H2B with XbaI and SpeI
		+	** In a 30 μL reaction, cut LOV with XbaI and SpeI
		+	** In a 30 μL reaction, cut a pSB1A2 plasmid (from Rene) XbaI and SpeI
		+
		+	{\| class="wikitable" border=1 cellpadding="5" cellspacing="0"
		+	\|-
		+	\| Reagent \|\| H2B\|\| LOV \|\| pSB1A2
		+	\|-
		+	\| DNA \|\| 8.0 \|\| 8.0 \|\| 15.0
		+	\|-
		+	\| XbaI \|\| 1.0\|\| 1.0 \|\| 1.0
		+	\|-
		+	\| SpeI \|\| 1.0 \|\| 1.0 \|\| 1.0
		+	\|-
		+	\| 10x buffer \|\| 3.0 \|\| 3.0 \|\| 3.0
		+	\|-
		+	\| dH<sub>2</sub>O \|\| --- \|\| --- \|\| ---
		+	\|-
		+	\| Total vol. \|\| 30. 0 \|\| 30.0 \|\| 30.0
		+	\|}
		+
		+
		+	* Run entire 30 μL on a 1% gel (use the big fat-tooth well comb)
		+	* Cut band out of gel (use UV box); H2B = ~410, LOV = ~461, pSB1A2 = ~2000
		+	* Extract DNA from gel

Vi Nguyen: /* November 12, 2012 */

2012-11-13T11:58:15Z

November 12, 2012

←Older revision		Revision as of 11:58, 13 November 2012
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	\| H2B \|\| 0.052 \|\| 0.028 \|\| 1.865 \|\| 51.607		\| H2B \|\| 0.052 \|\| 0.028 \|\| 1.865 \|\| 51.607
	\|-		\|-
-	\| LOV \|\| 0.048 \|\| 0.026 \|\| 1.86 \|\| 48.095	+	\| LOV \|\| 0.048 \|\| 0.026 \|\| 1.860 \|\| 48.095
	\|}		\|}

Vi Nguyen: /* November 12, 2012 */

2012-11-13T11:57:48Z

November 12, 2012

←Older revision		Revision as of 11:57, 13 November 2012
Line 15:		Line 15:
	\| H2B \|\| 0.052 \|\| 0.028 \|\| 1.865 \|\| 51.607		\| H2B \|\| 0.052 \|\| 0.028 \|\| 1.865 \|\| 51.607
	\|-		\|-
-	\| LOV 0.048 \|\| 0.026 \|\| 1.86 \|\| 48.095	+	\| LOV \|\| 0.048 \|\| 0.026 \|\| 1.86 \|\| 48.095
	\|}		\|}

Vi Nguyen: /* Entry title */

2012-11-13T11:57:18Z

Entry title

←Older revision		Revision as of 11:57, 13 November 2012
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	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==November 12, 2012==
-	* ~~Insert content here~~...	+	* The PCR products were purified with the Zymo DNA Clean and Concentration Kit
		+
		+	{\| class="wikitable" border=1 cellpadding="5" cellspacing="0"
		+	\|-
		+	\| PCR Product \|\| 260\|\| 280 \|\| 260/280 \|\| ng/μL
		+	\|-
		+	\| H2B \|\| 0.052 \|\| 0.028 \|\| 1.865 \|\| 51.607
		+	\|-
		+	\| LOV 0.048 \|\| 0.026 \|\| 1.86 \|\| 48.095
		+	\|}

Vi Nguyen: Autocreate 2012/11/12 Entry for Haynes_Lab:Notebook/Investigating_Photo-Switchable_Synthetic_Nucleosomes

2012-11-13T11:34:10Z

Autocreate 2012/11/12 Entry for Haynes_Lab:Notebook/Investigating_Photo-Switchable_Synthetic_Nucleosomes

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{|{{table}} width="800"
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

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