Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/12
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==November 12, 2012== | ==November 12, 2012== | ||
| - | * The PCR products were purified with the Zymo DNA Clean | + | * The PCR products were purified with the Zymo DNA Clean & Concentrator Kit |
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** In a 30 μL reaction, cut H2B with XbaI and SpeI | ** In a 30 μL reaction, cut H2B with XbaI and SpeI | ||
** In a 30 μL reaction, cut LOV with XbaI and SpeI | ** In a 30 μL reaction, cut LOV with XbaI and SpeI | ||
| - | ** In a 30 μL reaction, cut a pSB1A2 plasmid (from Rene) XbaI and SpeI | + | ** In a 30 μL reaction, cut a [http://partsregistry.org/Part:pSB1A2 pSB1A2 plasmid] (from Rene) XbaI and SpeI |
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November 12, 2012
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