Haynes Lab:Notebook/Inteins/2013/03/08: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2013/03/08 Entry for Haynes_Lab:Notebook/Inteins) |
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==Entry title== | ==Entry title== | ||
René: | |||
Added some cmp to some old cmp plates. Added 25ul liquid cmp stock to 225ul of water, added to plates, spread with beads, left on bench for about 2 hours then put at 4deg<br> | |||
transfered the colonies on amp intein plates to new amp plates using parafilm. kept the old ones just in case. if nothing grows, I'll try retransforming<br> | |||
picked colonies from B0034+C0061 (RBS+LuxI) and B0034+C0078 (RBS+LasI) plates (transformed yesterday from Fuqing). Growing in 4ml LB+amp.<br><br> | |||
KAH9 and KAH10 assembly:<br> | |||
Colony 2, the one that looked like it worked on the colony PCR, hasn't grown much after leaving the plate overnight in the incubator so it's out on my bench. | |||
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Revision as of 15:29, 8 March 2013
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Entry titleRené:
Added some cmp to some old cmp plates. Added 25ul liquid cmp stock to 225ul of water, added to plates, spread with beads, left on bench for about 2 hours then put at 4deg |