Haynes Lab:Notebook/Inteins/2013/03/01: Difference between revisions
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==Entry title== | ==Entry title== | ||
* | * Completed a restriction/digest from really good mini prep! | ||
*Used brand new enzymes for the restriction/ digest | |||
*Made a brand new gel | |||
1KBT KAH9(186) KAH9(160) KAH10 (116) KAH10 (106) | |||
*Put 10 of ladder, 30 in other wells. | |||
http://openwetware.org/wiki/Image:3-1-2013_gel.jpg | |||
*Run dna recovery kit | |||
**Melted @ 55.6 degrees C for 6 min., vortex in between | |||
**8uL + 7 water to elute dna in last step | |||
*Completed ligation/transformation | |||
*Used both 2x and 10x buffers | |||
*Get competent cells and keep on ice | |||
*Used both buffers to see if lab shared stock Roche ligation buffer was contaminated. | |||
*Used brand new ligase and ligation buffer | |||
*Left ligations for at least 30 mins. | |||
*Add 50 uL of competent cells | |||
Revision as of 15:00, 5 March 2013
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Entry title
1KBT KAH9(186) KAH9(160) KAH10 (116) KAH10 (106)
http://openwetware.org/wiki/Image:3-1-2013_gel.jpg
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