Haynes Lab:Notebook/Inteins/2013/02/19: Difference between revisions
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Amanda Ispas (talk | contribs) (Autocreate 2013/02/19 Entry for Haynes_Lab:Notebook/Inteins) |
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Entry title== | ==Entry title== | ||
* | * Completed a restriction/digest | ||
*Incubated @ 37.5- 37 degrees C for 20 mins. | |||
*Completed a gel | |||
*1KBT 9(186) 9(160) 10(116) 10(106) | |||
*8.5 of the ladder, 30 uL for dna wells | |||
*Also did a gel extraction | |||
**300 uL ADB Buffer of every gel slice | |||
**Incubate @ 50 degrees C for 3 min., vortex, put back for another 3 min., repeat until melted | |||
**Follow the gel dna recovery kit | |||
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Latest revision as of 22:31, 26 September 2017
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