Haynes Lab:Notebook/Inteins/2013/02/19

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(Autocreate 2013/02/19 Entry for Haynes_Lab:Notebook/Inteins)
Current revision (17:34, 5 March 2013) (view source)
(Entry title)
 
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==Entry title==
==Entry title==
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* Insert content here...
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* Completed a restriction/digest
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*Incubated @ 37.5- 37 degrees C for 20 mins.
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*Completed a gel
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*1KBT  9(186)  9(160)  10(116)  10(106)
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*8.5 of the ladder, 30 uL for dna wells
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*Also did a gel extraction
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**300 uL ADB Buffer of every gel slice
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**Incubate @ 50 degrees C for 3 min., vortex, put back for another 3 min., repeat until melted
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**Follow the gel dna recovery kit
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Entry title

  • Completed a restriction/digest
  • Incubated @ 37.5- 37 degrees C for 20 mins.
  • Completed a gel
  • 1KBT 9(186) 9(160) 10(116) 10(106)
  • 8.5 of the ladder, 30 uL for dna wells
  • Also did a gel extraction
    • 300 uL ADB Buffer of every gel slice
    • Incubate @ 50 degrees C for 3 min., vortex, put back for another 3 min., repeat until melted
    • Follow the gel dna recovery kit


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