Haynes Lab:Notebook/Inteins/2013/02/19
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==Entry title== | ==Entry title== | ||
| - | * | + | * Completed a restriction/digest |
| - | + | *Incubated @ 37.5- 37 degrees C for 20 mins. | |
| + | *Completed a gel | ||
| + | *1KBT 9(186) 9(160) 10(116) 10(106) | ||
| + | *8.5 of the ladder, 30 uL for dna wells | ||
| + | *Also did a gel extraction | ||
| + | **300 uL ADB Buffer of every gel slice | ||
| + | **Incubate @ 50 degrees C for 3 min., vortex, put back for another 3 min., repeat until melted | ||
| + | **Follow the gel dna recovery kit | ||
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