Haynes Lab:Notebook/Inteins/2013/02/19: Difference between revisions
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Amanda Ispas (talk | contribs) (Autocreate 2013/02/19 Entry for Haynes_Lab:Notebook/Inteins) |
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==Entry title== | ==Entry title== | ||
* | * Completed a restriction/digest | ||
*Incubated @ 37.5- 37 degrees C for 20 mins. | |||
*Completed a gel | |||
*1KBT 9(186) 9(160) 10(116) 10(106) | |||
*8.5 of the ladder, 30 uL for dna wells | |||
*Also did a gel extraction | |||
**300 uL ADB Buffer of every gel slice | |||
**Incubate @ 50 degrees C for 3 min., vortex, put back for another 3 min., repeat until melted | |||
**Follow the gel dna recovery kit | |||
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Revision as of 14:34, 5 March 2013
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