Haynes Lab:Notebook/Inteins/2013/02/15: Difference between revisions

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(Autocreate 2013/02/15 Entry for Haynes_Lab:Notebook/Inteins)
 
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==Entry title==
==Entry title==
* Insert content here...
* * Conducted a mini prep
 
**Began by pouring the cells from round-bottom tubes into 2mL microfuge tubes.
**I then spun down the cells in the centrifuge for 3 min., poured out the liquid waste  and spun again
**This leaves the 600 microliters to begin the mini prep protocol
**Used the Zyppy plasmid mini prep kit
**Also ran a gel
**I put KAH 9 KAH 9 KAH 10 KAH 10 into the gel





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Entry title

  • * Conducted a mini prep
    • Began by pouring the cells from round-bottom tubes into 2mL microfuge tubes.
    • I then spun down the cells in the centrifuge for 3 min., poured out the liquid waste and spun again
    • This leaves the 600 microliters to begin the mini prep protocol
    • Used the Zyppy plasmid mini prep kit
    • Also ran a gel
    • I put KAH 9 KAH 9 KAH 10 KAH 10 into the gel