Haynes Lab:Notebook/Inteins/2013/02/13

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  • Redid the restriction/digest on 9/10
  • Incubated for 20 mins.
  • Added 10 ul of the ladder, and 30 ul of the dna
  • Ran gel for more than 30 mins to run gel
  • KAH 9 is 687 bp
  • KAH 10 is NLS
  • Vo120 is 2400 bp for backbone
  • Cut out 10B for extraction
  • Do another restriction/digest for longer with the enzymes
  • http://openwetware.org/wiki/Image:2013-02-13_gel.jpg



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