Haynes Lab:Notebook/Inteins/2013/02/13

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(Autocreate 2013/02/13 Entry for Haynes_Lab:Notebook/Inteins)
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==Entry title==
==Entry title==
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* Insert content here...
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*Redid the restriction/digest on 9/10
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*Incubated for 20 mins.  
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*Added 10 ul of the ladder, and 30 ul of the dna
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*Ran gel for more than 30 mins to run gel
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*KAH 9 is 687 bp
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*KAH 10 is NLS
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*Vo120 is 2400 bp for backbone
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*Cut out 10B for extraction
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*Do another restriction/digest for longer with the enzymes
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* http://openwetware.org/wiki/Image:2013-02-13_gel.jpg

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Entry title

  • Redid the restriction/digest on 9/10
  • Incubated for 20 mins.
  • Added 10 ul of the ladder, and 30 ul of the dna
  • Ran gel for more than 30 mins to run gel
  • KAH 9 is 687 bp
  • KAH 10 is NLS
  • Vo120 is 2400 bp for backbone
  • Cut out 10B for extraction
  • Do another restriction/digest for longer with the enzymes
  • http://openwetware.org/wiki/Image:2013-02-13_gel.jpg



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