Haynes Lab:Notebook/Inteins/2013/02/13
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==Entry title== | ==Entry title== | ||
| - | * | + | *Redid the restriction/digest on 9/10 |
| + | *Incubated for 20 mins. | ||
| + | *Added 10 ul of the ladder, and 30 ul of the dna | ||
| + | *Ran gel for more than 30 mins to run gel | ||
| + | *KAH 9 is 687 bp | ||
| + | *KAH 10 is NLS | ||
| + | *Vo120 is 2400 bp for backbone | ||
| + | *Cut out 10B for extraction | ||
| + | *Do another restriction/digest for longer with the enzymes | ||
| + | * http://openwetware.org/wiki/Image:2013-02-13_gel.jpg | ||
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