Haynes Lab:Notebook/Inteins/2013/02/01
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< Haynes Lab:Notebook | Inteins | 2013 | 02(Difference between revisions)
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* Did restriction/ digest on KAH 9/ KAH 10 | * Did restriction/ digest on KAH 9/ KAH 10 | ||
| + | *Used both tubes KAH9/KAH 10A, KAH 9B, KAH 10B | ||
*Incubated for 20 mins. to ensure effectiveness | *Incubated for 20 mins. to ensure effectiveness | ||
| - | *Ran gel for 20 mins. | + | *Ran gel for 20 mins. AT 100V |
| + | * 1KBT 9A 10A 9B 10B | ||
| + | *Added 8ul of ladder, 15ul of dna | ||
*Gel was unsuccessful, so will have to redo. | *Gel was unsuccessful, so will have to redo. | ||
*http://openwetware.org/wiki/Image:2-1-2013_gel.JPG | *http://openwetware.org/wiki/Image:2-1-2013_gel.JPG | ||
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