Haynes Lab:Notebook/Inteins/2013/01/24

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  • Learned about BioBrick cloning
  • Completed a restriction/ digest
    • 15 microliters of plasmid
    • 1 microliter of restriction enzyme 1
    • 1 microliter of restriction enzyme 2
    • 3 microliter of 10x buffer
    • 10 microliters of water
  • check temperature first before running experiment

http://openwetware.org/wiki/Image:2013-01-24_gel.jpg


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