Haynes Lab:Notebook/Inteins/2013/01/24: Difference between revisions
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Amanda Ispas (talk | contribs) (Autocreate 2013/01/24 Entry for Haynes_Lab:Notebook/Inteins) |
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==Entry title== | ==Entry title== | ||
* | * Learned about BioBrick cloning | ||
*Completed a restriction/ digest | |||
---15 microliters of plasmid | |||
---1 microliter of restriction enzyme 1 | |||
---1 microliter of restriction enzyme 2 | |||
---3 microliter of 10x buffer | |||
--10 microliters of water | |||
**check temperature first before running experiment | |||
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Revision as of 19:38, 3 February 2013
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Entry title
---15 microliters of plasmid ---1 microliter of restriction enzyme 1 ---1 microliter of restriction enzyme 2 ---3 microliter of 10x buffer --10 microliters of water
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