Haynes Lab:Notebook/Inteins/2013/01/24
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==Entry title== | ==Entry title== | ||
| - | * | + | * Learned about BioBrick cloning |
| + | *Completed a restriction/ digest | ||
| + | |||
| + | ---15 microliters of plasmid | ||
| + | ---1 microliter of restriction enzyme 1 | ||
| + | ---1 microliter of restriction enzyme 2 | ||
| + | ---3 microliter of 10x buffer | ||
| + | --10 microliters of water | ||
| + | |||
| + | **check temperature first before running experiment | ||
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Revision as of 22:38, 3 February 2013
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---15 microliters of plasmid ---1 microliter of restriction enzyme 1 ---1 microliter of restriction enzyme 2 ---3 microliter of 10x buffer --10 microliters of water
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