Haynes Lab:Notebook/Inteins/2013/01/18: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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* Transform plasmids | * Transform plasmids | ||
warm plates for 10- 20 mins. | *warm plates for 10- 20 mins. | ||
add 9 microliters of water | *add 9 microliters of water | ||
add 1 microliters of plasmid | *add 1 microliters of plasmid | ||
add 20 microliters of bacterial cells- should be on ice | *add 20 microliters of bacterial cells- should be on ice | ||
add the 30 microliters to plates | *add the 30 microliters to plates | ||
sit in incubator over night | *sit in incubator over night | ||
Latest revision as of 22:24, 26 September 2017
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