Haynes Lab:Notebook/Inteins/2013/01/18
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==Entry title== | ==Entry title== | ||
| - | * | + | * Transform plasmids |
| + | |||
| + | *warm plates for 10- 20 mins. | ||
| + | *add 9 microliters of water | ||
| + | *add 1 microliters of plasmid | ||
| + | *add 20 microliters of bacterial cells- should be on ice | ||
| + | *add the 30 microliters to plates | ||
| + | *sit in incubator over night | ||
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