Haynes Lab:Notebook/Inteins/2013/01/18

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(Entry title)
Current revision (22:19, 3 February 2013) (view source)
(Entry title)
 
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* Transform plasmids  
* Transform plasmids  
-
warm plates for 10- 20 mins.
+
*warm plates for 10- 20 mins.
-
add 9 microliters of water
+
*add 9 microliters of water
-
add 1 microliters of plasmid
+
*add 1 microliters of plasmid
-
add 20 microliters of bacterial cells- should be on ice  
+
*add 20 microliters of bacterial cells- should be on ice  
-
add the 30 microliters to plates  
+
*add the 30 microliters to plates  
-
sit in incubator over night  
+
*sit in incubator over night  

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  • Transform plasmids
  • warm plates for 10- 20 mins.
  • add 9 microliters of water
  • add 1 microliters of plasmid
  • add 20 microliters of bacterial cells- should be on ice
  • add the 30 microliters to plates
  • sit in incubator over night



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