Haynes Lab:Notebook/Inteins/2013/01/18
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< Haynes Lab:Notebook | Inteins | 2013 | 01(Difference between revisions)
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* Transform plasmids | * Transform plasmids | ||
| - | warm plates for 10- 20 mins. | + | *warm plates for 10- 20 mins. |
| - | add 9 microliters of water | + | *add 9 microliters of water |
| - | add 1 microliters of plasmid | + | *add 1 microliters of plasmid |
| - | add 20 microliters of bacterial cells- should be on ice | + | *add 20 microliters of bacterial cells- should be on ice |
| - | add the 30 microliters to plates | + | *add the 30 microliters to plates |
| - | sit in incubator over night | + | *sit in incubator over night |
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