Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2016/02/09: Difference between revisions

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* DNA quantification
* DNA quantification
* Restriction digest, HA1_v0120 with E&S (106 bp)
* Restriction digest, HA1_v0120 with E&S (106 bp)
* Restriction digest, DBN011 with E&X (5216 bp)
* Restriction digest, DBN011 with E&X (5769 bp)
* DNA quantification
* DNA quantification
* ligation
* ligation

Revision as of 09:54, 9 February 2016

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Overview

DBN011 ligation results:

  • Negative control: 8 colonies
  • Treatment: 18 colonies

Going to pick 4 colonies from the treatment plate and proceed with streaks & liquid cultures.

To do:

  • plasmid prep
  • DNA quantification
  • Restriction digest, HA1_v0120 with E&S (106 bp)
  • Restriction digest, DBN011 with E&X (5769 bp)
  • DNA quantification
  • ligation
  • transformation


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