Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/11/30: Difference between revisions
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# Negative control - pJET vector only | # Negative control - pJET vector only | ||
# negative control - no plasmid | # negative control - no plasmid | ||
==Notes== | |||
May have to perform a PCR clean-up on the sample next time before transforming cells. | |||
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Revision as of 14:03, 30 November 2015
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OverviewPerforming LCR on the PNK-treated parts from last week. Methods
Thermal cycler program:
Transformation Follow the instructions in the protocol described here. Setting up three plates:
NotesMay have to perform a PCR clean-up on the sample next time before transforming cells. |