Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/11/24: Difference between revisions
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(Autocreate 2015/11/24 Entry for Haynes_Lab:Notebook/HPK-CFP_insertion_into_Gal4EED/Luc_using_CRISPR) |
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Today's project is...</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Today's project is...</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Overview== | ==Overview== | ||
Waiting for new primers to arrive. In the meantime, attempted ligation of LCR product into pJET vector followed by transformation into DH5α-Turbo competent E. coli. | |||
==Method== | |||
[http://openwetware.org/wiki/DNA_ligation#Method Ligation] | |||
Used 3.5 µL of LCR product and 50 ng of pJET vector. Total ligation volume 10 µL. | |||
[http://openwetware.org/wiki/Haynes:TransformationPlasmids#Traditional_Method:_Chemically_competent_cells_.2B_ligation Transformation] | |||
Two plates set up: sample containing cells transformed with ligation product, and negative control containing pJET only. Expect to see no colonies on pJET control due to kill gene. | |||
Latest revision as of 01:21, 27 September 2017
Today's project is... | Main project page Previous entry Next entry |
OverviewWaiting for new primers to arrive. In the meantime, attempted ligation of LCR product into pJET vector followed by transformation into DH5α-Turbo competent E. coli. MethodUsed 3.5 µL of LCR product and 50 ng of pJET vector. Total ligation volume 10 µL. Two plates set up: sample containing cells transformed with ligation product, and negative control containing pJET only. Expect to see no colonies on pJET control due to kill gene.
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