Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/05/20: Difference between revisions
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(Autocreate 2015/05/20 Entry for Haynes_Lab:Notebook/HPK-CFP_insertion_into_Gal4EED/Luc_using_CRISPR) |
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==Overview== | ==Overview== | ||
Ordered new primers for PCR amplification of the DBN006 fragment. They're now matched to have similar melting temperatures and have a larger anchor on either side of the BsaI binding site. Primer names are DBN006 f1 and DBN006 r1. | |||
Revision as of 09:41, 22 May 2015
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OverviewOrdered new primers for PCR amplification of the DBN006 fragment. They're now matched to have similar melting temperatures and have a larger anchor on either side of the BsaI binding site. Primer names are DBN006 f1 and DBN006 r1.
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