Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/04/30: Difference between revisions
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Incubate the ligation reaction in a thermocycler: | Incubate the ligation reaction in a thermocycler (program name DBN_IIS): | ||
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Revision as of 11:16, 30 April 2015
Ligation/Amplification of DBN002 & DBN003 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OverviewGoing to try Type IIS Digestion/Ligation of DBN002 & DBN003, followed by PCR amplification. Type IIS Digestion/Ligation
Incubate the ligation reaction in a thermocycler (program name DBN_IIS):
Clean-up of IIS LigationUse the Qiagen kit. If possible, final volume elution of 30 µL. PCR
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