Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/04/23: Difference between revisions
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<u>Colony count</u> | <u>Colony count</u><br> | ||
Negative control: 0 | Negative control: 0<br> | ||
Positive control (vector): hundreds | Positive control (vector): hundreds<br> | ||
Sample: 6-10 | Sample: 6-10<br> | ||
I picked six colonies, streaked them on a plate, and set up 5mL liquid cultures to incubate overnight at 37°C. Will perform plasmid minipreps tomorrow. | I picked six colonies, streaked them on a plate, and set up 5mL liquid cultures to incubate overnight at 37°C. Will perform plasmid minipreps tomorrow. | ||
Revision as of 18:37, 23 April 2015
 Transformation Results from DBN001_pSB1A3 Cloning
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OverviewColony count I picked six colonies, streaked them on a plate, and set up 5mL liquid cultures to incubate overnight at 37°C. Will perform plasmid minipreps tomorrow. | |
