Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/04/17: Difference between revisions
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Expected size fragments from EcoRI/SpeI digestion: 2132, 224 | Expected size fragments from EcoRI/SpeI digestion: 2132, 224 | ||
[[Image:2015-04-17_DBN001_pSB1A3_digest_annotated.png]] | |||
Still no trace of plasmid present. Going to assume cloning did not work. Will try again next week with fresh phosphatase & ligase, using dephosphorylated backbone as another control. | |||
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Revision as of 13:22, 17 April 2015
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Plasmid Miniprep of DBN001_pSB1A3 (repeat)--Notes-- Once again, liquid culture density is very low. Suspect it's because there's no actual plasmid present. --DNA Concentration--
--Restriction Digest--
Digest for 10 minutes at 37°C. --Gel Electrophoresis-- Lost most of sample 1 while pipetting into the lane. Bands will probably be very faint. Expected size fragments from EcoRI/SpeI digestion: 2132, 224
Still no trace of plasmid present. Going to assume cloning did not work. Will try again next week with fresh phosphatase & ligase, using dephosphorylated backbone as another control. | |||||||||||||||||||||||||||
