Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/04/17: Difference between revisions

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<b>--DNA Concentration--</b>
<b>--DNA Concentration--</b>


Sample ID Conc. (ng/uL) St Dev (ng/uL) 260/280
{| {{table}}
DBN001_pSB1A3 colony 1 78.3 4.98 1.89
| align="center" style="background:#f0f0f0;"|'''Sample ID'''
DBN001_pSB1A3 colony 2 59.9 4.37 2.14
| align="center" style="background:#f0f0f0;"|'''Conc. (ng/uL)'''
| align="center" style="background:#f0f0f0;"|'''St Dev (ng/uL)'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
|-
| DBN001_pSB1A3 colony 1||78.27||4.98||1.89
|-
| DBN001_pSB1A3 colony 2||59.95||4.37||2.14
|}


<b>--Restriction Digest--</b>
<b>--Restriction Digest--</b>

Revision as of 11:19, 17 April 2015

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Plasmid Miniprep of DBN001_pSB1A3 (repeat)

--Notes--

Once again, liquid culture density is very low. Suspect it's because there's no actual plasmid present.

--DNA Concentration--

Sample ID Conc. (ng/uL) St Dev (ng/uL) 260/280
DBN001_pSB1A3 colony 1 78.27 4.98 1.89
DBN001_pSB1A3 colony 2 59.95 4.37 2.14

--Restriction Digest--

Reagent Volume
DNA 600ng (7.7 - 10 µL)
10x FastDigest Buffer 3
EcoRI 1
PstI 1
Water 17.3 - 15
Total 30

Digest for 15 minutes at 37°C.



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