Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/04/15: Difference between revisions
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Digest for 15 minutes at 37°C. | Digest for 15 minutes at 37°C. | ||
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<b>--Gel Electrophoresis--</b> | |||
[[Image:2015-04-15_mCherry_vector_digest_annotated.png]] | |||
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Revision as of 18:21, 15 April 2015
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Transformation Results for mCherry vector + gBlock
I'm going to set up two cultures for the two colonies on the sample plate, but am not very confident of the success of the ligation given the colonies on the negative control. Plasmid Miniprep of mCherry Vector--DNA Concentration--
--Restriction Digest--
Digest for 15 minutes at 37°C.
--Gel Electrophoresis--
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