Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/03/25: Difference between revisions
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Gel image of yesterday's PCR: | Gel image of yesterday's PCR: | ||
[Image:2015-03-25_DBN_annotated.jpg] | [[Image:2015-03-25_DBN_annotated.jpg | 500px]] | ||
Even after extracting the double gene copy fragment using gel extraction and performing PCR on that fragment alone, there's still preferential annealing and amplification of the single copy fragment. I'm going to order a shorter forward primer to see if that makes any difference. | Even after extracting the double gene copy fragment using gel extraction and performing PCR on that fragment alone, there's still preferential annealing and amplification of the single copy fragment. I'm going to order a shorter forward primer to see if that makes any difference. | ||
Revision as of 11:14, 26 March 2015
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Entry titleGel image of yesterday's PCR:
Even after extracting the double gene copy fragment using gel extraction and performing PCR on that fragment alone, there's still preferential annealing and amplification of the single copy fragment. I'm going to order a shorter forward primer to see if that makes any difference. | |
