Haynes Lab:Notebook/Engineering PC-TFs/2015/05/26: Difference between revisions

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Revision as of 12:25, 21 May 2015



 		<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

Minipreps

Check OWW Entry for 5-18-15.

Assemblies

  1. KAH 001: hPCD/(S/E)/~186 + PLflex linker/(X/E)/2763
  2. KAH 002: hPCD/(S/E)/~186 + PLflex x4 linker/(X/E)/2931
  3. KAH 003: hPCD/(S/E)/~186 + PLrig linker/(X/E)/2763
  4. KAH 004: hPCD/(S/E)/~186 + PLrig x4 linker/(X/E)/2931

> Gel Extraction

Reagent Volume  
30 μL/lane, 1% gel
DNA (plasmid) ^ to 25μL
10x buffer 3.0
enzyme 1 1.0
enzyme 2 1.0
dH2O ---
  30μL 30μL --> 37°C/ ~30 min.


> Measure conc.'s

Sample OD260 260/280 ng/μL
1. KAH01(S/E)
2. KAH021(X/E)
3. KAH022(X/E)
4. KAH023(X/E)
5. KAH024(X/E)

> Classic Ligation https://docs.google.com/spreadsheets/d/1t8hsxoZxc4I5XKYOZ_LCsNwZpc1Csjrnt8JocjqsICM/edit?usp=sharing



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