Haynes Lab:Notebook/Engineering PC-TFs/2015/04/20: Difference between revisions
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|style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | |style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | ||
|style="background-color: #800000" align="center"| | |style="background-color: #800000" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Summary== | |||
* Restriction Digest/Gel Verification, Concentration Reading<br> | * Restriction Digest/Gel Verification, Concentration Reading<br> | ||
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No insertion: 268, 883, 1243, 2783 <br> <br> | No insertion: 268, 883, 1243, 2783 <br> <br> | ||
| rowspan="10" | | | rowspan="10" | [[Image:4-20-15.jpg|400px|]]<br> | ||
15 μL/lane; 1% agarose;<br> | 15 μL/lane; 1% agarose;<br> | ||
<u>Observed lengths:</u> <br> | <u>Observed lengths:</u> <br> | ||
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Note: Too much buffer was used. Messed up Well 7. | Note: Too much buffer was used. Messed up Well 7. As expected, "Bl01 LCR V3" and "BL01 LSR V4" are actually re-transformations of Bl09, they were just labeled incorrectly. | ||
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'''New PcTF Assembly'''<br> | |||
PCD, AD, RFP in V0120.<br> | |||
Either PCR or cut and paste biobrick assembly.<br> | |||
Biobrick assembly:<br> | |||
PCD: Cut out with Eco&Not<br> | |||
RFP: Cut out with Not&Spe<br> | |||
AD: Cut out with XbaI&PstI<br> | |||
Ligate and then cut with XbaI and SpeI to make compatible with MV10 insertion site. | |||
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Latest revision as of 00:55, 27 September 2017
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Summary
Digest and Gel Verification
New PcTF Assembly |