Haynes Lab:Notebook/Engineering PC-TFs/2015/02/14: Difference between revisions
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==Summary== | ==Summary== | ||
'''Transfection'''<br> | '''Transfection'''<br> | ||
# Brought 2.0 μg total plasmid DNA up to 20 μL total volume with fresh, sterile DNase-free water in fresh, sterile 1.5 mL tubes. | # Brought 2.0 μg total plasmid DNA up to 20 μL total volume with fresh, sterile DNase-free water in fresh, sterile 1.5 mL tubes. | ||
10μL BL01v(202ng/μL) + 10μL dH<sub>2</sub>O, 6μLBL09 (333ng/μL) + 14μL dH<sub>2</sub>O.<br> | 10μL BL01v(202ng/μL) + 10μL dH<sub>2</sub>O, 6μLBL09 (333ng/μL) + 14μL dH<sub>2</sub>O.<br> | ||
#Took the DNA samples to the tissue culture room. | #Took the DNA samples to the tissue culture room. |
Revision as of 10:43, 16 February 2015
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SummaryTransfection
10μL BL01v(202ng/μL) + 10μL dH2O, 6μLBL09 (333ng/μL) + 14μL dH2O.
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