Haynes Lab:Notebook/Engineering PC-TFs/2015/01/19: Difference between revisions
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*DNA in lanes 2-9 have relatively low concentration (<90ng/μL).<br> | *DNA in lanes 2-9 have relatively low concentration (<90ng/μL).<br> | ||
Wells 6&7 (BL01 traditional ligation) look promising; will sequence tomorrow. <br> | |||
First four lanes are empty yet again yet have reasonable concentration and purity in the plate reader. Degradation? <br> | |||
Using DD123 & DD122 primers, a forward insertion will result in a band of length 3371 running the PCR reaction. | Using DD123 & DD122 primers, a forward insertion will result in a band of length 3371 running the PCR reaction. | ||
Revision as of 19:13, 19 January 2015
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SummaryLigation Digest and Gel Verification
Wells 6&7 (BL01 traditional ligation) look promising; will sequence tomorrow. |