Haynes Lab:Notebook/Engineering PC-TFs/2015/01/19: Difference between revisions
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|style="background-color: #800000" align="center"| | |style="background-color: #800000" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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*DNA in lanes 2-9 have relatively low concentration (<90ng/μL).<br> | *DNA in lanes 2-9 have relatively low concentration (<90ng/μL).<br> | ||
Wells 6&7 (BL01 traditional ligation) look promising; will sequence tomorrow. <br> | |||
First four lanes are empty yet again yet have reasonable concentration and purity in the plate reader. Degradation? <br> | |||
Using DD123 & DD122 primers, a forward insertion will result in a band of length 3371 running the PCR reaction. | Using DD123 & DD122 primers, a forward insertion will result in a band of length 3371 running the PCR reaction. | ||
Latest revision as of 00:39, 27 September 2017
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SummaryLigation Digest and Gel Verification
Wells 6&7 (BL01 traditional ligation) look promising; will sequence tomorrow. |