Haynes Lab:Notebook/Engineering PC-TFs/2014/12/18: Difference between revisions

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(Autocreate 2014/12/18 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs)
 
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==Summary==
==Summary==
*  
* Protein melt with BSA
<br>
==Summary==


'''BSA Melting Curve Reagent Volumes'''
<br>
{| {{table}} border="1" cellspacing="3" <!-- Plates. table -->
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| rowspan="7" | [[Image:BSA melting curve reagent volumes 12-22-2014.PNG|600px|Hover name]]
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<br>
'''BSA Melting Curve Results'''
<br>
{| {{table}} border="1" cellspacing="3" <!-- Plates. table -->
|- valign="top"
| rowspan="7" | [[Image:BSA melting curve results.PNG|600px|Hover name]]
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<br>
Similar results when compared to the PcTF data. Beginning temperature for data collection could be too high.
<br>
Samples with highest amount of protein showed the highest intensity peaks at the beginning of data collection in this data set, which is expected and lends support to the idea that the samples were loaded into the plate incorrectly or were mislabeled for the PcTF data set.<br>
Buffer does not seem to have an effect on the data (HEPES against PBS).
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__NOTOC__


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Revision as of 12:33, 7 January 2015



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Summary

  • Protein melt with BSA


Summary

BSA Melting Curve Reagent Volumes

Hover name


BSA Melting Curve Results

Hover name


Similar results when compared to the PcTF data. Beginning temperature for data collection could be too high.
Samples with highest amount of protein showed the highest intensity peaks at the beginning of data collection in this data set, which is expected and lends support to the idea that the samples were loaded into the plate incorrectly or were mislabeled for the PcTF data set.
Buffer does not seem to have an effect on the data (HEPES against PBS).


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