Haynes Lab:Notebook/Engineering PC-TFs/2014/12/02: Difference between revisions

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| rowspan="10" | [[Image:20140901 180224.jpg|400px|Today's gel]]<br>
| rowspan="10" | [[|400px|Today's gel]]<br>
15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]<br>  
15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]<br>  


Revision as of 21:58, 6 December 2014



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Summary

  • BL01, BL05, CMV/MV9 Purification AGAIN (insert purity unacceptable at last attempt).
Reagent Volume

Expected:
CMV/MV9 (Well 1): 5200
BL01 (Well 3): 2500, 3300
BL05 (Well 5): 2500, 3300


 [[|400px|Today's gel]]

15 μL/lane; 1% agarose; Ladder

Observed lengths:

DNA(CMV/MV9, BL01, BL05) 15.0 μL
10X buffer 3.0 μL
XbaI 2.0 μL
SpeI (no SpeI for CMV/MV9) 2.0 μL
dH2O 8 (10 for CMV/MV9) μL
Total 30 μL --> 37°C/ 30 min.




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