Haynes Lab:Notebook/Engineering PC-TFs/2014/12/02: Difference between revisions

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==Summary==
==Summary==
*  
* BL01, BL05, CMV/MV9 Purification AGAIN (insert purity unacceptable at last attempt).<br>
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Revision as of 16:54, 2 December 2014



<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • BL01, BL05, CMV/MV9 Purification AGAIN (insert purity unacceptable at last attempt).
Reagent Volume

Expected:
CMV/MV9 (Well 1): 5200
BL01 (Well 3): 2500, 3300
BL05 (Well 5): 2500, 3300


Today's gel

15 μL/lane; 1% agarose; Ladder

Observed lengths:

DNA(CMV/MV9, BL01, BL05) 15.0 μL
10X buffer 3.0 μL
XbaI 2.0 μL
SpeI (no SpeI for CMV/MV9) 2.0 μL
dH2O 8 (10 for CMV/MV9) μL
Total 30 μL --> 37°C/ 30 min.