Haynes Lab:Notebook/Engineering PC-TFs/2014/10/13: Difference between revisions

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# U2OS BL09  = 181
# U2OS BL09  = 181
# U2OS (no transfection) = 25
# U2OS (no transfection) = 25
# BL09 negative ctrl (no DNA) = 34
# BL09 negative ctrl (no protein) = 34




Revision as of 09:46, 16 October 2014



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Summary

  • RFP
  • Bradford

Plate reader measurements

RFP signal

  1. U2OS BL09 = 181
  2. U2OS (no transfection) = 25
  3. BL09 negative ctrl (no protein) = 34


Bradford assay

  1. Standard 1 (0 μg) = 0.276
  2. Standard 2 (1 μg) = 0.234
  3. Standard 3 (2 μg) = 0.394
  4. Standard 4 (4 μg) = 0.443
  5. Standard 5 (8 μg) = 0.644
  6. Standard 6 (16 μg) = 0.829
  7. U2OS BL09 = 0.313
  8. U2OS (no transfection) = 0.302
  9. BL09 negative = 0.048

Standard curve


Final results:

Sample Protein μg/μL Protein*100 μL Raw Fluor. (100 uL) Fluor/ μg protein
U2OS BL09 0.765 76.50 181 2.365929
U2OS (no transfection) 0.464 46.45 25 0.538235
BL09 negative -6.47 -647.54 34 -0.05251



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