Haynes Lab:Notebook/Engineering PC-TFs/2014/10/13: Difference between revisions

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==Summary==
==Summary==
*  
* RFP
* Bradford


'''Plate reader measurements'''
'''Plate reader measurements'''
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* Results:
* Results:


# BL09 1 = 181
# U2OS BL09 = 181
# BL09 2 (no transfection) = 25
# Mock Transfection = 25
# BL09 negative ctrl (no DNA) = 34
 




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# Standard 5 (8 μg) = 0.644
# Standard 5 (8 μg) = 0.644
# Standard 6 (16 μg) = 0.829
# Standard 6 (16 μg) = 0.829
# BL09 1 = 0.31
# U2OS BL09 = 0.313
# BL09 2 (no transfection) = 0.3
# Mock Transfection = 0.302
# BL09 negative = 0.047
 


Standard curve
Standard curve


[[Image:CG091514_Bradford.jpg|400px]]
[[Image:Bradford rfp.png|600px]]




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| Sample||Protein μg/μL||Protein*100 μL||Raw Fluor. (100 uL)||Fluor/ μg protein
| Sample||Protein μg/μL||Protein*100 μL||Raw Fluor. (100 uL)||Fluor/ μg protein
|-
|-
| BL09 1||10.42||1041.96||109||0.104610111
| U2OS BL09||0.765||76.50||181||2.365929
|-
| BL09 2||10.20||1019.64||610||0.598248687
|-
|-
| BL09 negative||9.75||975.00||47||0.048205128
| Mock Transfection||0.464||46.45||25||0.538235
|}
|}


Latest revision as of 00:25, 27 September 2017



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Summary

  • RFP
  • Bradford

Plate reader measurements

RFP signal

  1. U2OS BL09 = 181
  2. Mock Transfection = 25


Bradford assay

  1. Standard 1 (0 μg) = 0.276
  2. Standard 2 (1 μg) = 0.234
  3. Standard 3 (2 μg) = 0.394
  4. Standard 4 (4 μg) = 0.443
  5. Standard 5 (8 μg) = 0.644
  6. Standard 6 (16 μg) = 0.829
  7. U2OS BL09 = 0.313
  8. Mock Transfection = 0.302


Standard curve


Final results:

Sample Protein μg/μL Protein*100 μL Raw Fluor. (100 uL) Fluor/ μg protein
U2OS BL09 0.765 76.50 181 2.365929
Mock Transfection 0.464 46.45 25 0.538235



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