Haynes Lab:Notebook/Engineering PC-TFs/2014/10/11: Difference between revisions
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'''LCR'''<br> | '''LCR'''<br> | ||
Oligo bridges initially | Oligo bridges initially 30nm. | ||
Diluted down to 30nM in 25μL reaction volume (Added 300μL H<sub>2</sub>O to IDT tube with pelleted DNA to yield 100μM, added 3μL of that dilution to 97μL H<sub>2</sub>O to yield 3μM, added 5μL of that dilution to 45μL H<sub>2</sub>O to yield 300nM, added 2.5μL of this dilution to the 25μL reaction volume for LCR).<br> | Diluted down to 30nM in 25μL reaction volume (Added 300μL H<sub>2</sub>O to IDT tube with pelleted DNA to yield 100μM, added 3μL of that dilution to 97μL H<sub>2</sub>O to yield 3μM, added 5μL of that dilution to 45μL H<sub>2</sub>O to yield 300nM, added 2.5μL of this dilution to the 25μL reaction volume for LCR).<br> | ||
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Revision as of 19:44, 11 October 2014
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Summary
Purified BL09 Negative Control Protein Extract
Digest
LCR
|