Haynes Lab:Notebook/Engineering PC-TFs/2014/09/15: Difference between revisions
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* Centrifuged samples at max rpm for five minutes in cold room. | * Centrifuged samples at max rpm for five minutes in cold room. | ||
* Labeled three new 1.5mL tubes. | * Labeled three new 1.5mL tubes. | ||
* Transferred | * Transferred supernatant to new tube being careful not to disturb the pellet at the bottom of each tube. | ||
* Used in Bradford Assay and stored in the -20°C. | |||
Revision as of 13:42, 16 September 2014
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Summary
Protein extraction from transfected cells
Plate reader measurements RFP signal
Standard curve
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