Haynes Lab:Notebook/Engineering PC-TFs/2014/09/13: Difference between revisions
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DNA for transfection: <br><br> | DNA for transfection: <br><br> | ||
BL09 Ligation 2: 111.303ng/μL <br> | BL09 Ligation 2: 111.303ng/μL <br> | ||
Used 17.97μL DNA + ~2μL dH<sub>2</sub>O for transfection of well one.<br><br> | Used 17.97μL DNA + ~2μL dH<sub>2</sub>O for transfection of cells in well one.<br><br> | ||
BL09 Ligation 5: 486.51ng/μL<br> | BL09 Ligation 5: 486.51ng/μL<br> | ||
Used 4.11μL DNA + ~15.89μL dH<sub>2</sub>O for transfection of well two. | Used 4.11μL DNA + ~15.89μL dH<sub>2</sub>O for transfection of cells in well two. | ||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 17:35, 13 September 2014
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SummaryPerformed Lipid-mediated transfection of DNA protocol.
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