Haynes Lab:Notebook/Engineering PC-TFs/2014/09/13: Difference between revisions

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DNA for transfection: <br><br>
DNA for transfection: <br><br>
BL09 Ligation 2: 111.303ng/μL <br>
BL09 Ligation 2: 111.303ng/μL <br>
Used 17.97μL DNA + ~2μL dH<sub>2</sub>O for transfection of well one.<br><br>
Used 17.97μL DNA + ~2μL dH<sub>2</sub>O for transfection of cells in well one.<br><br>
BL09 Ligation 5: 486.51ng/μL<br>
BL09 Ligation 5: 486.51ng/μL<br>
Used 4.11μL DNA + ~15.89μL dH<sub>2</sub>O for transfection of well two.
Used 4.11μL DNA + ~15.89μL dH<sub>2</sub>O for transfection of cells in well two.


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Revision as of 17:35, 13 September 2014



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Summary

Performed Lipid-mediated transfection of DNA protocol.

DNA for transfection:

BL09 Ligation 2: 111.303ng/μL
Used 17.97μL DNA + ~2μL dH2O for transfection of cells in well one.

BL09 Ligation 5: 486.51ng/μL
Used 4.11μL DNA + ~15.89μL dH2O for transfection of cells in well two.