Haynes Lab:Notebook/Engineering PC-TFs/2014/09/13: Difference between revisions

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==Summary==
==Summary==
Performed [http://openwetware.org/wiki/Haynes:TransfectionPlasmid_Lipo Lipid-mediated transfection of DNA protocol]. <br>
Performed [http://openwetware.org/wiki/Haynes:TransfectionPlasmid_Lipo Lipid-mediated transfection of DNA protocol]. <br><br>
DNA for transfection: <br><br>
DNA for transfection: <br><br>
BL09 Ligation 2: 111.303ng/μL <br>
BL09 Ligation 2: 111.303ng/μL <br>
Used 17.97μL DNA + ~2μL dH<sub>2</sub>O for transfection of well one.<br><br>
Used 17.97μL DNA + ~2μL dH<sub>2</sub>O to obtain 2μg DNA and 20μL total volume for transfection of cells in well one.<br><br>
BL09 Ligation 5: 486.51ng/μL<br>
BL09 Ligation 5: 486.51ng/μL<br>
Used 4.11μL DNA + ~15.89μL dH<sub>2</sub>O for transfection of well two.
Used 4.11μL DNA + ~15.89μL dH<sub>2</sub>O to obtain 2μg DNA and 20μL total volume for transfection of cells in well two.


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Latest revision as of 00:17, 27 September 2017



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Summary

Performed Lipid-mediated transfection of DNA protocol.

DNA for transfection:

BL09 Ligation 2: 111.303ng/μL
Used 17.97μL DNA + ~2μL dH2O to obtain 2μg DNA and 20μL total volume for transfection of cells in well one.

BL09 Ligation 5: 486.51ng/μL
Used 4.11μL DNA + ~15.89μL dH2O to obtain 2μg DNA and 20μL total volume for transfection of cells in well two.



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