Haynes Lab:Notebook/Engineering PC-TFs/2014/09/01: Difference between revisions
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<u><b>Expected:</b></u> | <u><b>Expected:</b></u> | ||
<br> | <br> | ||
<br>BL01(Well: 4-8, | <br>BL01(Well: 4-8, 11-14), BL05(Well:2) <br> | ||
Forward insertion: 710, 7010. <br> | Forward insertion: 710, 7010. <br> | ||
Reverse insertion: 3230, 4490. <br> | Reverse insertion: 3230, 4490. <br> | ||
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| rowspan="10" | [[ | | rowspan="10" | [[Image:20140901 180224.jpg|400px|Today's gel]]<br> | ||
15 μL/lane; 1% agarose;<br> | 15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]<br> | ||
<u>Observed lengths:</u> <br> | <u>Observed lengths:</u> <br> | ||
W2 | W2(BL05Lig1): 5000, 700; <br> | ||
W4(BL01Lig1): 3200, 4500; <br> | |||
W4( | W5(BL01Lig2): 5000, 700; <br> | ||
W5( | W6(BL01Lig3): 5000, 700; <br> | ||
W6( | W7(BL01Lig4): 5000, 700; <br> | ||
W7( | W8(BL01Lig5): 5000, 700; <br> | ||
W8( | W9(Empty CMV/MV9): 5000, 700; <br> | ||
W9( | W11(BL01Lig6): 5000, 700; <br> | ||
W12(BL01Lig7): 5000, 700; <br> | |||
W13(BL01Lig8): 5000, 700;<br> | |||
W14(BL01Lig9): 5000, 700; <br> | |||
|- | |- | ||
| DNA(BL01x2, BL05x1, BL09x2, CMV/MV9x1) || 2.0 μL | | DNA(BL01x2, BL05x1, BL09x2, CMV/MV9x1) || 2.0 μL | ||
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| Total || 15 μL --> 37°C/ 30 min. | | Total || 15 μL --> 37°C/ 30 min. | ||
|} | |} | ||
<br> | |||
One reverse insertion. No forward insertions. Will reattempt ligation. | |||
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Revision as of 17:48, 2 September 2014
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Summary
Restriction Digest/Gel Verification
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