Haynes Lab:Notebook/Engineering PC-TFs/2014/08/05: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2014/08/05 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs)
 
(fix raw html notebook nav)
 
(3 intermediate revisions by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span>
|style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span>
|style="background-color: #800000" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #800000" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Summary==
==Summary==
*
'''Picked Colonies for Liquid Cultures'''
<br> CMV/MV9, BL01, BL05, BL09, RhlI plates had growth while the negative control had no growth.<br>


{| {{table}} border="1" cellspacing="3" <!-- Plates. table -->
|- valign="top"
| rowspan="7" | [[Image:20140805 150836.jpg|400px|Hover name]] <br>
<b>ALL PcTF COLONIES WERE PICKED</b><br>
<u>From left to right, top to bottom:</u><br>
Experimental plate BL01: One colony.<br>
Experimental plate BL05: Four colonies. <br>
Experimental Plate Bl09: Six colonies.<br>
CMV/MV9 Backbone: Ignore. Did not use the proper plasmid for benchmarking.<br>
Positive control RhlI: Lots of growth. Cells seem good.<br>
Negative control: No growth. Plates seem good.
|}
<br>
Grew liquid cultures of the 11 PcTF colonies using 4mL amp resistant LB liquid media.<br>
Put in incubator at 37°C and 238RPM for overnight growth.


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
 


__NOTOC__
__NOTOC__

Latest revision as of 00:10, 27 September 2017



 		Main project page
Previous entry      Next entry

Summary

Picked Colonies for Liquid Cultures
CMV/MV9, BL01, BL05, BL09, RhlI plates had growth while the negative control had no growth.

Hover name

ALL PcTF COLONIES WERE PICKED
From left to right, top to bottom:
Experimental plate BL01: One colony.
Experimental plate BL05: Four colonies.
Experimental Plate Bl09: Six colonies.
CMV/MV9 Backbone: Ignore. Did not use the proper plasmid for benchmarking.
Positive control RhlI: Lots of growth. Cells seem good.
Negative control: No growth. Plates seem good.


Grew liquid cultures of the 11 PcTF colonies using 4mL amp resistant LB liquid media.
Put in incubator at 37°C and 238RPM for overnight growth.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Engineering_PC-TFs/2014/08/05&oldid=1015073"