Haynes Lab:Notebook/Engineering PC-TFs/2014/07/28: Difference between revisions
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{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | {| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | ||
|- valign="top" | |- valign="top" | ||
| rowspan="7" | [[Image:Pctf gel extraction 20140728 180402.jpg|400px|Hover name]]<br> 0.8% agarose; cut the brightest band furthest down at ~ 2500bp; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | | rowspan="7" | [[Image:Pctf gel extraction 20140728 180402.jpg|400px|Hover name]]<br> 0.8% agarose; cut the brightest band furthest down at ~ 2500bp&1400bp; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|} | |} | ||
* Used Zymo gel purification kit. | * Used Zymo gel purification kit. | ||
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* Pipetted 200μL wash buffer to the tube, centrifuged at max rpm for 30 seconds. Repeated once. | * Pipetted 200μL wash buffer to the tube, centrifuged at max rpm for 30 seconds. Repeated once. | ||
* Transferred column to new labeled 1.5mL tube. | * Transferred column to new labeled 1.5mL tube. | ||
* Pipetted 15μL | * Pipetted 15μL dH<sub>2</sub>O to the column. | ||
* Spun at max rpm for 30 seconds. | * Spun at max rpm for 30 seconds. | ||
Revision as of 13:22, 29 July 2014
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Summary
Ligation Digest and Gel Verification (2)
Gel Extraction/Purification
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