Haynes Lab:Notebook/Engineering PC-TFs/2014/07/28: Difference between revisions
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|style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | |style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | ||
|style="background-color: #800000" align="center"| | |style="background-color: #800000" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
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{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | {| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | ||
|- valign="top" | |- valign="top" | ||
| rowspan="7" | [[Image:Pctf gel extraction 20140728 180402.jpg|400px|Hover name]]<br> 0.8% agarose; cut the brightest band furthest down at ~ 2500bp; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | | rowspan="7" | [[Image:Pctf gel extraction 20140728 180402.jpg|400px|Hover name]]<br> 0.8% agarose; cut the brightest band furthest down at ~ 2500bp&1400bp; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
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* Used Zymo gel purification kit. | * Used Zymo gel purification kit. | ||
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* Pipetted 200μL wash buffer to the tube, centrifuged at max rpm for 30 seconds. Repeated once. | * Pipetted 200μL wash buffer to the tube, centrifuged at max rpm for 30 seconds. Repeated once. | ||
* Transferred column to new labeled 1.5mL tube. | * Transferred column to new labeled 1.5mL tube. | ||
* Pipetted 15μL | * Pipetted 15μL dH<sub>2</sub>O to the column. | ||
* Spun at max rpm for 30 seconds. | * Spun at max rpm for 30 seconds. | ||
Latest revision as of 00:08, 27 September 2017
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Summary
Ligation Digest and Gel Verification (2)
Gel Extraction/Purification
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