Haynes Lab:Notebook/Engineering PC-TFs/2014/07/08: Difference between revisions

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==Summary==
==Summary==
*
'''Ligation Colonies'''
 
----
<br>
{| {{table}} border="1" cellspacing="3" <!-- Plates. table -->
|- valign="top"
| rowspan="7" | [[Image:1BL0120140708 174328 (1).jpg|400px|Hover name]] <br> BL01. Four colonies.
|- valign="top"<br>
| rowspan="7" | [[Image:2BL0520140708 174335.jpg|400px|Hover name]]<br> BL05. Five colonies.
|}
{| {{table}} border="1" cellspacing="3" <!-- Plates. table -->
|- valign="top"
| rowspan="7" | [[Image:3BL0910502508 10204136250987662 5495498411586337712 n.jpg|400px|Hover name]] <br> BL09. Nine satellite colonies.
|- valign="top"<br>
| rowspan="7" | [[Image:4 cmvmv9 10509665 10204136250707655 7668079819192103144 n.jpg|400px|Hover name]] <br> Dephos CMV/MV9 Backbone. One colony.
|}
{| {{table}} border="1" cellspacing="3" <!-- Plates. table -->
|- valign="top"
| rowspan="7" | [[Image:5rhlI pos ctrl10494874 10204136250467649 979957952418741824 n.jpg|400px|Hover name]] <br> RhlI positive control. No colonies.
|- valign="top"
| rowspan="7" | [[Image:6negctrl10479177 10204136250187642 6640818424941448315 n.jpg|400px|Hover name]] <br> Negative control. No colonies.
|}
<br>
Picked three colonies from each PcTF plate. <br>Used 5ml media for liquid cultures. <br>Incubated overnight.


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Latest revision as of 00:05, 27 September 2017



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Summary

Ligation Colonies


Hover name
BL01. Four colonies.
Hover name
BL05. Five colonies.
Hover name
BL09. Nine satellite colonies.
Hover name
Dephos CMV/MV9 Backbone. One colony.
Hover name
RhlI positive control. No colonies.
Hover name
Negative control. No colonies.


Picked three colonies from each PcTF plate.
Used 5ml media for liquid cultures.
Incubated overnight.


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