Haynes Lab:Notebook/Engineering PC-TFs/2014/06/16: Difference between revisions

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{| {{table}} border="1" cellspacing="3" <!-- Plates. table -->
{| {{table}} border="1" cellspacing="3" <!-- Plates. table -->
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| rowspan="7" | [[Image:20140609 172345.jpg|400px|Hover name]] <br> [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
| rowspan="7" | [[Image:20140616 154617.jpg|400px|Hover name]] <br> [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
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Revision as of 13:33, 17 June 2014



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Summary

Digest All the Things Addendum

Well One: Ladder
Well Two

Reagent Volume Expected:
2520(insert)+4968(V0120 Vector) = 7488bp
Observed:

DNA(BL05 M.P) 8.0 μL
10X buffer 1.5 μL
XbaI 1.0 μL
dH2O 4.5 μL
Total 15 μL --> 37°C/ 30 min.


Well Three

Reagent Volume Expected:
2520(insert)+4968(V0120 Vector) = 7488bp
Observed:

DNA(BL05A) 4.0 μL
10X buffer 1.5 μL
XbaI 1.0 μL
dH2O 8.5 μL
Total 15 μL --> 37°C/ 30 min.

Well Four

Reagent Volume Expected:
1431 (insert) + 4968 (V0120 Vector) = 6399bp
Observed:

DNA(BL09 M.P.) 8.0 μL
10X buffer 1.5 μL
XbaI 1.0 μL
dH2O 4.5 μL
Total 15 μL --> 37°C/ 30 min.


Well Five

Reagent Volume Expected:
1431 (insert) + 4968 (V0120 Vector) = 6399bp
Observed:

DNA(BL09B) 4.0 μL
10X buffer 1.5 μL
XbaI 1.0 μL
dH2O 8.5 μL
Total 15 μL --> 37°C/ 30 min.


Well Six

Reagent Volume Expected:
5197bp
Observed:

DNA(Phosphorylated CMV/MV9 ) 2.0 μL
10X buffer 1.5 μL
dH2O 11.5 μL
Total 15 μL --> 37°C/ 30 min.


Gel Verification

Hover name
Ladder

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