Haynes Lab:Notebook/Engineering PC-TFs/2014/05/24: Difference between revisions

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==Summary==
==Summary==
'''Miniprepped the three colonies' liquid cultures'''
'''Miniprepped the three colonies' liquid cultures'''<br>
*Eluted with 75μL dH<sub>2</sub>O.
Eluted with 75μL dH<sub>2</sub>O.<br>
----
----
'''Concentration'''
'''Concentration'''

Revision as of 12:45, 24 May 2014



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Summary

Miniprepped the three colonies' liquid cultures
Eluted with 75μL dH2O.

Concentration

Plasmid OD260 OD260/280 ng/μL
1. CMV/MV9 BL01 Ligation C1 0.17 1.899 170.143
1. CMV/MV9 BL01 Ligation C2 0.168 1.898 167.513
1. CMV/MV9 BL01 Ligation C3 0.201 1.885 200.8

Verification Strategy
Check with XbaI and NruI

Hover name
Length of forward insertion.
Hover name
Length of reverse insertion.
Hover name
Length of no insertion.


Length of no insertion cut and forward insertion cut are the same; no helpful enzymes are present in the restriction enzyme box in the -20°C. PCR verification with DD 123B and DD 122B should allow for verification.


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