Haynes Lab:Notebook/Engineering PC-TFs/2014/05/21: Difference between revisions
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{| class="wikitable" width=400px | {| class="wikitable" width=400px | ||
| || Ligation || Negative Control | | || <u>Ligation</u> || <u>Negative Control</u> | ||
|- | |- | ||
| Insert DNA (X ng) || 9μL || '''none''' | | Insert DNA (X ng) || 9μL || '''none''' | ||
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| || 20.0 μL total || same | | || 20.0 μL total || same | ||
|- | |- | ||
| colspan="3" | | | colspan="3" | | ||
|} | |} | ||
*Mix the reaction(s) thoroughly by flicking the tube. | |||
*Incubate at room temperature overnight. | |||
Revision as of 17:08, 21 May 2014
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SummarySetting up the ligation (CMV/MV9 & BL01)
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