Haynes Lab:Notebook/Engineering PC-TFs/2014/05/12: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 64: Line 64:
|}
|}


*Put in Cameron box labeled Dephos XbaI CMV/MV9 5/12/14
*Eluted with 15μL (-1μL for concentration reading) H<sub>2</sub>0 and put in Cameron box labeled Dephos XbaI CMV/MV9 5/12/14






__NOTOC__
__NOTOC__

Revision as of 15:02, 13 May 2014



 		<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • Using colony one of previously constructed CMV/MV9.
  • Restriction Digest
DNA(CMV/MV9) Concentration: 163ng/μL 5.0 μL
10X buffer 1.5 μL
XbaI 1.0 μL
dH2O 7.5 μL
Total 15 μL --> 37°C/ 15 min.
Mixture from Above Digest 15.0 μL
10X Phosphotase buffer 2.0 μL
Phosphotase 1.0 μL
dH2O 2.0 μL
Total 20 μL --> 37°C/ 10 min. 75°C for 2 min.


  • DNA Purification Kit standard protocol. Used 600μL binding buffer solution.
Plasmid OD260 OD260/280 ng/μL
1. Purified DNA 0.059 2.046 59.108
  • Eluted with 15μL (-1μL for concentration reading) H20 and put in Cameron box labeled Dephos XbaI CMV/MV9 5/12/14
Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Engineering_PC-TFs/2014/05/12&oldid=793249"