Haynes Lab:Notebook/Engineering PC-TFs/2013/10/25: Difference between revisions
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| KAH126||281.975 ng/μL | | KAH126||281.975 ng/μL | ||
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{| {{table}} cellspacing="3" <!-- Digest rxn. table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | '''Reagent''' | |||
| bgcolor=#cfcfcf | '''Volume''' | |||
| rowspan="7" | [[Image:kah126gel.jpg|250px| Diagnostic digest of KAH126]] <br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | |||
|- | |||
| DNA (plasmid) || 20.0 μL | |||
|- | |||
| XbaI|| 1.0 μL | |||
|- | |||
| PstI|| 1.0 μL | |||
|- | |||
| Green 10x FastDigest buffer || 3.0 μL | |||
|- | |||
| dH<sub>2</sub>O || 5 μL | |||
|- | |||
| || 30 μL --> 37°C/ ~10 min. | |||
|} | |} | ||
Revision as of 20:18, 25 October 2013
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October 25, 2013Sigma-Aldrich Mini-prep Kit
Measure concentration of each fragment:
|